Table 1.

Primers for amplification of predicted and 16S rRNA genes

Target fragments	Forward primer sequence (5ʹ → 3ʹ)	Reverse primer sequence (5ʹ → 3ʹ)	Function annotation
EstP1	GTGAAGCCGTGGCGTTGT	GTGCGGTTTGCTGTAGGG	Beta-phosphoglucomutase
EstP2	ACGCCACAAACCTGCTCC	CGCCGCCTGTATTCCAAA	Beta-phosphoglucomutase
EstP3	TCCAGGCGTTTGGCATTA	CGACACCGTGCTCTTCGT	Beta-phosphoglucomutase
EstP4	ATCAGGTCGGCGAGTATTT	GCGGTTATCCCAGTTGTT	Glucose-6-phosphate 1-dehydrogenase
EstP5	AGCCTTACGGTACGACTTCC	ACGCCGCTTTGTTTCTGC	Putative hydrolase
EstP6	TATGGCTACAACCGTCCTG	ATCACCGTGGATCACCAG	Pimeloyl- [acyl-carrier protein] methyl ester esterase
EstP7	GAGGCGTTGTTTGAGGCA	CAGGGTCTGGGCTTTGATT	2-Hydroxy-6-ketonona-2,4-dienedioic acid hydrolase
EstP8	CTGGCGACTATTCCCTCAA	CGTAAAGCCCATACCACAAC	Monoterpene epsilon-lactone hydrolase
EstP9	GTCGATCTGCGAGAACCG	CCGCCTTAGCAAACACCA	Pimeloyl- [acyl-carrier protein] methyl ester esterase
EstP10	GCGTGCTGGTGCTGGAAA	GACTGGTTGTGGCGGTGA	Gentisate 1,2-dioxygenase
EstP11	TTCTGTTTAGGCGTGGGAGC	CATGTTGTAGGACACGGCAAG	2,3-Dihydroxyphenylpropionate 1,2-dioxygenase
EstP12	CTGCTGGAATTTCCGTTTG	GCATTGCCCATTGCTGTT	4-Carboxymuconolactone decarboxylase
EstP13	GTCGGCATTTATCGTATGG	GTGATGGCAATCGGAAGA	4-Hydroxybenzoate decarboxylase
16S rRNA	CGCTACCATGCAGTCGAACG	GGTCCCCCTCTTTGGTCTTG	–