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. 2022 Apr 4;54(4):493–502. doi: 10.1038/s12276-022-00746-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Effects of tomatidine treatment on the morphology of hESC-CMs. a Bright field images of the control CMs and Toma-CMs on Day 30. Scale bar = 100 μm. b Quantification of the cell area of the control CMs and Toma-CMs. c–f Immunocytochemistry images (c) and quantification of the measurements of the perimeter (d), sarcomere length (e), and circularity (f) of the control CMs and Toma-CMs (n = 77). Representative TEM images (g) and quantification of the measurement of the z disk length (h) in the control CMs and Toma-CMs. Data are shown as the mean ± S.D. (n = 33). *p < 0.05; ^‡p < 0.01; ^#p < 0.001.