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. 2022 May 6;8:253. doi: 10.1038/s41420-022-01044-y

Fig. 3. NUAK2 does not enhance ferroptosis by depleting glutathione.

Fig. 3

a Relative glutathione levels (oxidized plus reduced forms) from BT-549 cells 72 h after transfection with either NUAK1-targeting, NUAK2-targeting, or nontargeting siRNA (control) and 24 h treatment with either vehicle or 20 µM buthionine sulfoximine (BSO) (n = 3 independent experiments). b Relative glutathione levels from BT-549 cells exogenously expressing NUAK2 or eGFP (controls) 24 h after treatment with either vehicle (untreated) or 20 µM BSO. (n = 4 technical replicates). Glutathione levels were normalized to total viable cells (CellTiter-Glo). Error bars denote standard deviation centered on the mean. The numbers above the brackets are p values from Student’s t-tests (two-sided), ns denotes not significant.