Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 6;13:2516. doi: 10.1038/s41467-022-30259-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a–d Percentage of nuclei with XIST RNA coats per colony in hESC lines UM63-1 (a, c) and UM77-2 (b, d) cultured in parallel in XF medium (a, b) and mTeSR1 medium (c, d) on HFFs. hESCs cultured with mTeSR1 medium displayed a significant decrease in nuclei with XIST RNA coating compared to hESCs cultured in XF medium during passaging (general linear model comparison; p < 0.001). All hESCs in this experiment were cultured in 5% O₂ on HFFs. The quantification data for P13-14 in b are taken from Fig. 3b. At least 100 nuclei were counted per colony for hESC RNA FISH quantification. The total number of colonies quantified at each passage range between 10 and 180 and are cataloged in source data. Source data are provided as a Source Data file.