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. 2022 May 6;13:2516. doi: 10.1038/s41467-022-30259-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–c Percentage of nuclei with XIST RNA coating in colonies of hESC line UM90-14 cultured in XF medium (a), XF medium supplemented with 0.98 mM LiCl (XF with LiCl) (b), and mTeSR1 medium (c). hESCs cultured in XF medium did not display a significant decrease in nuclei with XIST RNA coats across passaging (general linear model, p = 0.2). hESCs cultured in XF medium with LiCl and mTeSR1 medium lost XIST RNA coating in a significant percentage of nuclei per colony during passaging compared to cells cultured in XF medium (general linear model, p < 0.001). All hESCs in this experiment were cultured in 5% O₂ on HFFs. At least 100 nuclei were counted per colony for hESC RNA FISH quantification. The total number of colonies quantified at each passage range between 5 and 78 and are cataloged in source data. Source data are provided as a Source Data file.