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. 2022 May 6;5:423. doi: 10.1038/s42003-022-03377-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Immunofluorescence images of endogenous MFN2. Confocal microscopy analysis of HeLa cells expressing COX4-EGFP (mitochondria), immunostained for MFN2 (Alexa Fluor 555) and peroxisomal protein ABCD3 (Alexa Fluor 647). Scale bars: 5 μm. b The zoomed-in images of the white box in (a). White arrows point the places where ABCD3 overlaps with MFN. Scale bars: 1 μm. c Histograms display measured fluorescence intensity along the white line in the merge panels in b, with the cyan line represents mitochondria, the magenta line represents endogenous MFN2, and the red line represents peroxisome. d–f Immunofluorescence images and analysis of endogenous MFN1. Similar experiments were performed as in (a–c).