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. 2022 May 6;12:7442. doi: 10.1038/s41598-022-11070-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CFPs and OCT line scans from three different eyes and their corresponding pseudocolored images (derived from multispectral image analysis) showcasing the different types of drusen spectral theme classes that emerged. (A): An example of an eye where a single drusen spectral theme classes (red) emerged from the pseudocolored image. (B): An example where two separable spectral theme classes for drusen (pink and peach) emerged which were then pooled for the quantification of drusen area. (C): An example of an eye which yielded multiple spectral theme classes in the pseudocolored image for drusen (orange, light pink, and peach) as well as other AMD lesions such as pigmentary abnormalities (dark pink, maroon) that can be seen on the CFP and OCT (colored arrows indicate each spectral theme class relative to OCT line scan). Only the spectral theme classes identified as drusen were pooled for the quantification of drusen area (final row). Boxes in CFP and multispectral images indicate locations of magnified images. Lines through magnified CFP images indicate location of OCT line scan.