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. 2022 May 6;13:2514. doi: 10.1038/s41467-022-30298-4

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative live-cell images of parental histones H3.3-SNAP signals at the indicated time points in WT (n = 61), Pole4 KO (n = 66), Mcm2-2A (n = 66), Pola1-2A (n = 74) mES single and Mcm2-2A Pole4 KO double mutant (n = 72) cell lines. Purple channels are 647-SiR labeled-parental H3.3-SNAP, green channels are for mAG tagged-geminin expression. Scale bar, 10 µm. Tracked cells were circled. b The average time from G1/S to mitosis for WT, Pole4 KO, Mcm2-2A, Pola1-2A, and Mcm2-2A Pole4 KO double mutant mES cell lines based on the appearance of mAG tagged-geminin (G1/S) and the reduction of mAG tagged-geminin signals (mitosis). c–g Quantification of H3.3-SNAP signals at G1/S and G2 of each mother cell and G1 of its two daughter cells in WT, Pole4 KO, Mcm2-2A, Pola1-2A, and Mcm2-2A + Pole4 KO mutant mES cell lines. The cell cycle stage of each cell was based on mAG tagged-geminin signals. h Comparison of the relative amount of parental H3.3-SNAP at individual cells at late G2 in WT, Pole4 KO, Mcm2-2A, Pola1-2A and Mcm2-2A + Pole4 KO mES cell lines. The center line, the box limits and the whiskers were defined as described in Fig. 2c. b–g Data are presented as means ± SD. a–h n number of cells from two independent experiments. c–h Statistical analysis was performed by two-tailed unpaired Student t test with the P values marked on the graphs, ns no significant difference.