Figure 3.

Sequences of Chitinase G₀ larvae edited by embryo injection aligned to wildtype (WT)

(A) Summary of deep sequencing data of animals injected with sgRNA 1. Indel percentage is on the Y axis. The X axis depicts predicted Cas9 cut site (position 0) and sequences up- and downstream of the cut site.

(B) Representative sequences containing deletions corresponding to sgRNA 1. The predicted cut site is shown by a black arrow and dotted line. PAM site is indicated by a red rectangle outline. Alignments were generated using Snapgene.

(C) Forward (F) and Reverse (R) chromatograms of WT, CH5, and CH6 larvae. Additional sequences, chromatograms, and indel/substitution percentages calculated by deep sequencing are shown in Figure S5 and Table S4.