Figure 5.

Sequences of Proboscipedia G₀ larvae CRISPR edited by ReMOT Control aligned to wildtype (WT)

(A) Map depicting aligned homozygous and heterozygous deletions.

(B) Representative chromatogram alignment of WT and two homozygous mutants (R4 and R6). GC-rich flanking regions are underlined in blue. PAM sites are indicated by a red rectangle. The approximate cut sites are shown by black arrows and dotted lines. PAM sites are indicated by a red rectangle. The approximate cut sites are shown by black arrows. Alignments were generated using Snapgene.