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. 2022 May 6;13:2497. doi: 10.1038/s41467-022-30103-2

Fig. 4. Evaluation of treatments in a hindlimb ischemia model on ob/ob mice.

Fig. 4

A Representative mice foot images taken by laser speckle imaging. B Relative blood flow recovery after hind limb ischemia surgery on ob/ob mice (n = 11 for tmSCF, 12 for other groups). p = 0.0424 on tmSCFPL vs control, and p = 0.0495 on tmSCFND vs control. Kruskal–Wallis test with Dunn’s post hoc was used. C Immunostaining for PECAM on mice on the muscles from the ischemic calf and thigh muscle of the mice after 14 days. Scale bar = 100 µm. D Quantification small blood vessels in the calf and thigh muscle counted from PECAM immunostaining images (n = 7). p = 0.0116 on tmSCFND vs alginate. E The number of large blood vessels in the tissues (n = 7). p = 0.008 on tmSCFPL vs control and p = 0.009 on tmSCND vs control on calf muscle. p = 0.0035 on tmSCFPL vs control and p = 0.0122 on tmSCFND vs control on thigh muscle. Two-sided one-way ANOVA with Dunnett’s post hoc test was used. * indicates p value < 0.05, ** < 0.01, and *** <0.001. All the error bars are SEM.