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. 2022 Jan 17;3(1):101089. doi: 10.1016/j.xpro.2021.101089

Table 2.

Gradient used during mass spectrometry analysis of phospho-enriched samples

LC gradient for phospho-enriched samples
Time Duration % of buffer B Flow (nL/min)
0 min 0 min 2 300
2 min 2 min 2 300
122 min 120 min 25 300
132 min 10 min 35 300
142 min 10 min 95 300