Figure 9.

GINS2 promotes HCC progression in vivo. LM3 cells infected by sh-GINS2 or negative control sh-NC lentiviruses were implanted into nude mice and tumor growth was recorded. A-C. Images of tumors from different groups of nude mice. Tumor volume and average weight were assessed. D. IHC analysis of GINS2 and Ki-67 in the tumors derived from mice. E. GINS2 stable knockdown LM3 cells were injected into the tail vein of nude mice to induce lung metastasis. The relative luciferase activity of HCC tumor in nude mice from the sh-NC or sh-GINS2 group were determined using a live imaging system. Liver metastatic nodules were subjected to HE staining as indicated. All data are presented as mean ± S.E.M. *P < 0.05; **P < 0.01; ***P < 0.001.