TABLE 2.
Aminoglycoside cell uptake
| Antibiotic and cell type | Incubation time | Ea (μg/ml) | C/Eb | Reference |
|---|---|---|---|---|
| Streptomycin | ||||
| Kupffer cells | 30 min | 1 | <1 | 130 |
| Mouse peritoneal MPs | 1 h | 5 | <0.1 | 21 |
| Mouse peritoneal MPs | 2–4 h | 2 | <1 | 22 |
| Mouse peritoneal MPs | 20 h | 30 | <0.1 | 21 |
| Mouse peritoneal MPs | 24 h | 2 | ∼2 | 22 |
| Mouse peritoneal MPs | 48 h | 2 | ∼3.6 | 22 |
| Guinea pig peritoneal MPs | 48 h | 2 | >3 | 22 |
| BHK-21 | 48 h | 2 | >3 | 22 |
| Rat embryo fibroblasts | 3 days | 100 | 2.4 | 177 |
| Rat embryo fibroblasts | 4 days | 500 | 2 | 179 |
| Gentamicin | ||||
| Rabbit alveolar MPs | 1 min | 18 | 0.27 | 93 |
| Human PMNs | 15 min | 18 | 1.03 | 135 |
| Rabbit alveolar MPs | 15 min | 18 | 0.48 | 93 |
| Human PMNs | 30 min | 18 | 0.73 | 135 |
| Rabbit alveolar MPs | 30 min | 18 | 0.39 | 93 |
| Human PMNs | 60 min | 18 | 0.83 | 135 |
| Rabbit alveolar MPs | 60 min | 18 | 0.56 | 93 |
| Human PMNs | 120 min | 18 | 0.84 | 135 |
| Rabbit alveolar MPs | 120 min | 18 | 0.56 | 93 |
| Rat embryo fibroblasts | 4 days | 100 | 6 | 178 |
| Rat embryo fibroblasts | 4 days | 500 | 5.2 | 179 |
| Kanamycin | ||||
| Rat embryo fibroblasts | 4 days | 100 | 2.6 | 178 |
| Rat embryo fibroblasts | 4 days | 500 | 2.4 | 179 |
| Amikacin | ||||
| Rat embryo fibroblasts | 4 days | 500 | 3.6 | 179 |
a
E, antibiotic concentration used in the cell incubation medium.
b
C/E, ratio of the intracellular concentration of the antibiotic to the extracellular one.