Fig. 4. (i) Bright field (left), fluorescence (middle) and overlay (right) images of (a–c) HeLa cells treated with 5 μM N-CBT for 30 min, (d–f) HeLa cells treated with 5 μM N-CBT for 30 min followed by the addition of 20 μM ONOO⁻, (g–i) macrophages cells treated with 5 μM N-CBT for 30 min, (j–l) macrophages stimulated with 1 μg mL⁻¹ LPS and 50 ng mL⁻¹ IFN-γ for 12 h, and then 10 nM PMA for 30 min before incubated with 5 μM N-CBT for 30 min. (ii) Fluorescence intensity of fluorescence images b, e, h and k, the results are presented as mean ± standard deviation (n ≥ 8).