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Organization of the region containing antibiotic resistance genes in SXTMO10 and in V. cholerae O139 strains sensitive to Tm, Su, and Cm. The gene order found in strains 2055 and HKO139-SXTS (bottom) is compared to that of SXTMO10 (top). Homologous recombination between the identical sequences in orf2 and orf2′ may have resulted in loss of the antibiotic resistance genes. Also shown are the primers (LEFTF3, LEND4, and RUMA) used to amplify this region in 2055 and HKO139-SXTS.
