Figure 5.

VHH Z70 blocks intra-cellular seeding of Tau MTBD in HEK293 biosensor cells

(A and B) Analysis of Tau seeding in HEK293 Tau RD P301S FRET Biosensor cells. (A) Percentage of FRET positive cells in the whole population determined from flow cytometry data for cells transfected with vehicle or transfected with vehicle and VHH F8-2 followed by MTBD seeds (n = 3). (B) Percentage of FRET positive cells in the mCherry-gated population determined from flow cytometry data for cells transfected with VHH F8-2, VHH E4-1, and VHH Z70 followed by MTBD seeds (n = 3). A significant decrease of FRET signal, reporting a decrease intra-cellular MTBD aggregation, is observed in the presence of VHH Z70. ∗p value <0.05, ∗∗∗p value < 0.001, ns, not significant. Error bars: SEM.