Table 1.
List of the primary and secondary antibodies used for the study and of their working dilutions
| Antibody | Manufacturer | Host species | Working dilution |
|
|---|---|---|---|---|
| wb | IHC | |||
| α-syn (5G4, MABN389) | Cell Signaling | Rabbit | 1:400 | |
| α-syn (syn1, 610787) | BD | Mouse | 1:1,000 | 1:500 |
| β-actin (251001) | Synaptic System | Mouse | 1:10,000 | |
| DAT (sc-32258) | Santa Cruz | Rat | 1:500 | |
| GAPDH (G8795) | Sigma-Aldrich | Mouse | 1:7,000 | |
| GFP (ab183734) | Abcam | Rabbit | 1:500 | |
| Syn III (106,303) | Synaptic System | Rabbit | 1:3,000 | 1:600 |
| Syn II (106,203) | Rabbit | 1:3,000 | ||
| Syn I (106,103) | Rabbit | 1:3,000 | 1:600 | |
| TH (AB152) | Millipore | Rabbit | 1:2000 | 1:600 |
| anti-mouse IgG Cy3-conjugated | Jackson ImmunoResearch | Goat | 1:1,000 | |
| anti-rabbit IgG Cy3-conjugated | Goat | 1:1,000 | ||
| anti-rat IgG Cy3-conjugated | Goat | 1:1,000 | ||
| anti-rat IgG Alexa 647-conjugated | Goat | 1:800 | ||
| anti-rabbit IgG Alexa 647-conjugated | Goat | 1:1,000 | ||
| biotinylated anti-rabbit IgG | Vector Laboratories | Goat | 1:1,000 | |
| anti-rabbit IgG-HRP | Promega | Goat | 1:3,000 | |
| anti-mouse IgG-HRP | Goat | 1:3,000 | ||