Figure 3.

Overexpression of miR-486 inhibits cardiomyocyte apoptosis

(A) RT-PCR for miR-486 expression in primary cultured neonatal rat cardiomyocytes (NRCMs) treated with oxygen-glucose deprivation/reperfusion (OGDR) (n = 4). (B) TUNEL staining for α-actinin-labeled NRCMs transfected with miR-486 mimic, inhibitor, or negative control (NC) under OGDR stress (n = 4). Scale bar, 100 μm. (C and D) Western blot for Bax/Bcl-2 ratio and cleaved-caspase-3/caspase-3 ratio in OGDR-treated NRCMs transfected with miR-486 mimic (C), inhibitor (D), or negative controls (n = 6). (E) RT-PCR for miR-486 expression in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) treated with OGDR (n = 3). (F) TUNEL staining for cTnT-labeled human cardiomyocytes treated with OGDR (n = 3 technique repeats). Scale bar, 100 μm. Data between two groups were compared by unpaired two-tailed Student's t test. Data among three groups were compared by one-way ANOVA. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.