Figure 8.

β-catenin and METTL3 are involved in in vivo dissemination of cancer cells

(A) IHC-stained (β-catenin, E-Cad, E2F1, and c-Met), paraffin-embedded sections obtained from HeLa and HeLa M3^Mut/− cells. The scale bar represents 100 μm. (B) Expression levels of CTNNB1, CDH1, MET, and METTL3 mRNA in primary tumors and metastasized lung tumors isolated from MMTV-PyMT mice were detected by quantitative real-time PCR. (C) Protein levels of β-catenin, E-Cad, c-Met, and METTL3 in primary tumors and metastasized lung tumors isolated from MMTV-PyMT mice were detected by western blot analysis. (D) IHC-stained (METTL3 and β-catenin), paraffin-embedded sections obtained from primary tumors and metastasized lung tumors of MMTV-PyMT mice. The scale bar represents 50 μm. (E) Expression levels of CDH1, CTNNB1, METTL3, and MET in BT549^F0 and BT549^LMF3 cells were detected by quantitative real-time PCR. (F) Expression levels of E-Cad, β-catenin, METTL3, and c-Met in BT549^F0 and BT549^LMF3 cells were detected by western blot. (G) Relative mRNA expression of CTNNB1 in metastasized or non-metastasized lung cancers from Oncomine datasets. (H) Relative mRNA expression of CTNNB1 in stage I, II, III, and IV of lung cancer from Oncomine datasets. (I) Kaplan-Meier survival curves of OS based on E2F1 expression in individuals with lung cancer from the TCGA DB. (J) Kaplan-Meier survival curves of OS based on MET expression in individuals with lung cancer from the TCGA DB. Data in (B) and (E) are presented as mean ± SD from three independent experiments. Student’s t test; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared with Con. A representative from a total of three independent experiments is shown for (A) and (D).