Fig. 2. Optimization improves the responsiveness of glucose sensor. (A and B) Truncation mutants of Pro294/Tyr295 (FGBP3.1 μM) (A) and their fluorescence response (B) toward 100 mM glucose. The most responsiveness mutant N3C4 named FGBP27 μM is indicated in red boxes. (C) Titration curve of FGBP27 μM. Fluorescence ratios were normalized to the control condition in the absence of glucose. Data are presented in three biological replicates, and error bars represent SEM.
