Fig. 4. Effect of pH on FGBP1 mM and cpYFP. (A) Fluorescence intensities of FGBP1 mM and cpYFP with excitation at 400 nm or 485 nm, and emission at 528 nm. (B) pH-dependency of the excitation ratio 485/400 of FGBP1 mM and cpYFP. For A and B, data are normalized to the fluorescence or ratio at pH 7.4, and are presented in three biological replicates, and error bars represent SEM.
