Properties of five glucose sensorsa,b.
Sensor | Engineering | R sat/Rapo (%) | K d (mM) | Detection range (mM) |
---|---|---|---|---|
FGBP3.1 μM | P294/Y295 | 210 | 0.0031 | 0.0005–0.02 |
FGBP27 μM | P294/Y295, N3C4 | 590 | 0.027 | 0.0009–0.86 |
FGBP380 μM | P294/Y295, N3C4, N256S | 510 | 0.38 | 0.014–10 |
FGBP1 mM | P294/Y295, N3C4, L238S | 690 | 1.0 | 0.026–38 |
FGBP3.2 mM | P294/Y295, N3C4, A213R | 630 | 3.2 | 0.09–110 |
P294/Y295, cpYFP was inserted between Pro294 and Tyr295 amino acids of GGBP.
N3C4, N-terminal 3 amino acids and C-terminal 4 amino acids of cpYFP were truncated.