Figure 2.

TUG1 epigenetically regulates TET3 and the DUSP family by recruiting SUV39H1

(A and B) HTR-8/SVneo trophoblasts were transfected with siCon or siSUV39H1 for 48 h (n = 3). The silencing of SUV39H1 promoted the expression of downstream genes at both mRNA (A) and protein (B) levels. (C) HTR-8/SVneo cells were transfected with siTUG1 or siCon. ChIP coupled with qPCR was performed at 48 h after transfection. The mean relative enrichment of SUV39H1 over input after normalization against IgG (negative control) is shown (n = 3). ChIP coupled with qPCR showed that SUV39H1 and H3K9me3 were enriched in the promoter regions of TET3, DUSP2, DUSP4, and DUSP5, and the enrichment was decreased after silencing of TUG1. Error bars indicate mean ± SEM. ∗p < 0.05 and ∗∗p < 0.01.