Figure 4.

Hypoxia-induced miR-218 inhibits the expression of FOXP1

(A) HTR-8/SVneo cells were cultured under hypoxic conditions (0.5% oxygen) for 24 h. Quantitative real-time PCR was used to detect the mRNA expressions of HIF-1α and miR-218. (B and C) ChIP assay showed that HIF-1α was enriched at two sites of the promoter region of miR-218, and the enrichment was increased in HTR-8/SVneo cells exposed to 0.5% oxygen for 24 h. (D) Predicted base-pairing interaction between FOXP1 mRNA and miR-218. (E) Luciferase reporter assay was used to detect the interaction between miR-218 and FOXP1 in 293 and HTR-8/SVneo cells. Relative luciferase activity was normalized to Renilla luciferase activity. (F) Quantitative real-time PCR of genes of interest in HTR-8/SVneo cells exposed to 0.5% oxygen for 24 h (n = 3). Error bars indicate mean ± SEM. ∗p < 0.05 and ∗∗p < 0.01.