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. 2022 Feb 4;30(4):1692–1705. doi: 10.1016/j.ymthe.2022.01.043

Figure 5.

Figure 5

MiR-218 regulates the expression of FOXP1 at the post-transcriptional level

(A–C) Quantitative real-time PCR and western blot analysis of genes of interest in HTR-8/SVneo cells transfected with iCon or miR-218 inhibitor for 48 h (n = 3). (E–G) Quantitative real-time PCR and western blot analysis of genes of interest in HTR-8/SVneo cells transfected with miCon or mimics miR-218 for 48 h (n = 3). (H–J) HTR-8/SVneo cells were transfected with miCon + pCDNA, miR-218 + pCDNA, or miR-218 + pFOXP1 for 48 h. Total RNA and protein were extracted and analyzed using quantitative real-time PCR (left panel) and western blot (right panels), respectively (n = 3). (K–N) HTR-8/SVneo cells were transfected with iCon + siCon, inhibitor miR-218 + siCon, or inhibitor miR-218 + siFOXP1 for 48 h. Total RNA and protein were isolated and analyzed using quantitative real-time PCR (left panel) and western blot (right panels), respectively (n = 3). Data are from three independent experiments. Error bars indicate mean ± SEM. ∗p < 0.05 and ∗∗p < 0.01.