Skip to main content
. 2022 Feb 4;30(4):1692–1705. doi: 10.1016/j.ymthe.2022.01.043

Figure 6.

Figure 6

Transcription factor FOXP1 activates the transcription of TUG1 and inhibits the expression of TET3 and the DUSP family.

(A and B) Quantitative real-time PCR and western blot were performed to assess the expression of target genes in HTR-8/SVneo cells transfected FOXP1 plasmid (pFOXP1) or empty vector (pCDNA) for 48 h (n = 3). (C and D) Quantitative real-time PCR and western blot analysis of target genes in HTR-8/SVneo cells transfected with siCon or siTET3 for 48 h (n = 3). The putative binding sites of FOXP1 in the TUG1 promoter were predicted by JASPAR. The potential binding sites were highlighted in blue (E). Luciferase reporter assay (F) was used to determine the interaction between FOXP1 and TUG1. The luciferase activity was normalized to Renilla luciferase activity. (G) HTR-8/SVneo cells were transfected with siFOXP1 or siCon for 48 h. ChIP coupled with qPCR was then performed. The mean relative enrichment of FOXP1 over input after normalization against IgG (negative control) is shown (n = 3). (H) Upstream regulation of TUG1. Error bars indicate mean ± SEM. ∗p < 0.05 and ∗∗p < 0.01.