Fig. 2. Effect of RFX on cell death in ER+ mammary gland cancer. (A) RFX mediated activation of mitochondrial associated protein signalling in mammary gland cells. Protein extracted from control (1), toxic control (MNU, 47 mg kg⁻¹) (2), RFX (25 mg kg⁻¹) (3) and RFX (50 mg kg⁻¹) (4) were subjected to immunoblotting of pro-apoptotic (BAD) and anti-apoptotic (BCL-2 and BCL-xl), and inflammatory marker (NF-κβ). β-Actin was used as internal loading control. Each experiment was performed in triplicate; (B) respective quantitative densitometry graphs of immunoblots of the proteins; (C) and (D) regulation of caspase-3 and caspase-8 by RFX in serum samples respectively. The activity of caspase was determined by commercial fluorescence based kits; and (E) ratio of BAD/BCL-2 subjected to MNU and RFX treatment. The data were represented as mean ± SD of three independent experimental protocols. Comparisons were made on the basis of the one-way ANOVA followed by Bonferroni multiple test. All groups were compared to the toxic control and control groups (*^/ap < 0.05, **^/bp < 0.01, ***^/cp < 0.001).