Fig. 1. Schematic illustration of the assembly of a 5 × 5 pillar array for tumor spheroid culture and cryosection. (A) Assembly of 5 × 5 pillars at 9 mm distance for 3D cell cultures in 96-well format. Cross-sectional view of the pillars placed into the 96-well plate is also shown. (B) Assembly of 5 × 5 pillars at 3.2 mm distance on the pillar holder for embedding and cryosectioning. Each pillar has a diameter of 2 mm.
