Map of waterbodies and ancestry found for walleye (Sander vitreus) included in the present study. Cedar Bluff Reservoir, Kansas, USA, represents walleye in an entirely stocked population of unknown origin. Lake Manitoba and Lake Winnipeg (Manitoba, Canada) walleye represent native populations, although Lake Winnipeg has been stocked by Lake Manitoba walleye throughout the 20th century. In addition, floods in 1882, 1902, 1904, 2011, and 2014 have carried water from Lake Manitoba to Lake Winnipeg, and may have contributed to walleye gene flow. Pie charts represent estimated ancestry proportions with Admixture on n = 345 walleye total from K = 3 groups. Each pie chart corresponds to a sampling site, with the Red River, Matheson Island, and the Dauphin River in Lake Winnipeg, Swan Creek Hatchery in Lake Manitoba, and Cedar Bluff Reservoir in Kansas