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. 2022 May 7;20:219. doi: 10.1186/s12951-022-01443-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Self-assembly and characterization of ds-MIRNA/SPc complex. a Gel electrophoresis assay of ds-MIR164b retardation by SPc. The ds-MIR164b/SPc complexes (ds-MIRNA: 3 μg) were prepared at the mass ratios of 5:1, 3:1, 1:1 and 1:3 (ds-MIRNA: SPc), respectively. b Size distribution measurement of ds-MIR164b/SPc complex at the mass ratio of 1:1. c Zeta potential of ds-MIR164b/SPc complex at the mass ratio of 1:1. The “***” indicates the significant difference according to independent t-test at P < 0.001. d TEM images of ds-MIR164b/SPc complex at the mass ratio of 1:1. Small complexes were enlarged