Fig. 3.

Intracellular AIBP is located in the mitochondria of macrophages and inhibits inflammation. A Western blot was used to determine AIBP levels in the nuclei and mitochondria of THP-1-derived macrophages and BMDMs (n = 3). B Live cell imaging of BMDMs using a confocal microscope. Mitochondria were stained with MitoTracker Green. Scale bar = 2 μm. C Western blot analysis of AIBP levels in isolated mitochondria and BMDMs (n = 3). D qPCR analysis of the mRNA levels of inflammatory cytokines in BMDMs. E qPCR analysis of the mRNA levels of M1 and M2 marker genes in infected BMDMs treated with LPS + IFN-γ or IL-4 (n = 5). Data are presented as the means ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. One-way ANOVA followed by the Newman–Keuls test