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. 2022 May 7;20:60. doi: 10.1186/s12964-022-00858-8

Fig. 5.

Fig. 5

Mitochondrial AIBP maintains the macrophage phenotype by altering mitophagy through mitochondrial OXPHOS. A The ratio of mitochondrial DNA (mtDNA) to nuclear DNA (nucDNA) in AIBP−/−, AIBPWT or AIBP (ΔMLS) BMDMs was evaluated using qPCR (n = 5). B Western blot analysis of LC3b, p62 and TOM20 expression in BMDMs. C Live cell imaging of BMDMs using a confocal microscope. Mitochondria were stained with MitoTracker Green. Scale bar = 2 μm. D Western blot analysis of the expression of LC3b, p62 and TOM20 in BMDMs after treatment with mitochondrial division inhibitor 1 (mdivi-1). E qPCR analysis of the mRNA levels of M1 marker genes in BMDMs stimulated with LPS + IFN-γ after the Mdivi1 pretreatment and M2 marker genes in BMDMs stimulated with IL-4 after the Mdivi1 pretreatment (n = 5). Data are presented as the means ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. One-way ANOVA followed by the Newman–Keuls test