Fig. 6.
AIBP deficiency inhibits mitophagy via PINK1 cleavage. A Western blot analysis of the levels of Parkin and PINK1 in the total lysate of BMDMs (n = 5). B qPCR analysis of the Parkin and PINK1 mRNA levels in BMDMs (n = 5). C Western blot analysis of PINK1 and Parkin levels in the total lysate of BMDMs (n = 5). D–E The interaction of AIBP with TIM23 in 293 T cells was assessed by performing coimmunoprecipitation and Western blot assays. F qPCR was performed to detect the mRNA levels of M1 markers and M2 markers in MPMs extracted from AIBP−/−, AIBPWT or AIBP(ΔMLS) mice in the LV-control (control) group and LV-PINK1 (PINK1) group that were separately stimulated with LPS + IFN-γ and IL-4 (n = 5). Data are presented as the means ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. One-way ANOVA followed by the Newman–Keuls test