Fig. 4.

Functional analyses demonstrate BIN1 facilitates inflammation-induced cytokine production, as well as phagocytosis, in primary microglial cultures. A-B Bin1 siRNA treatment did not affect cytokine secretion in unchallenged microglial cultures. LPS exposure increased secretion, which was attenuated by the KD of Bin1. C-D Flow cytometric analysis of the fluorescent microsphere phagocytosis found that Bin1 reduction impeded the phagocytic capacity of primary microglia, both unchallenged and following LPS stimulation. E Phagocytosis of fibrillar Aβ₄₂ was unaffected by Bin1 silencing. *, p < 0.05; **, p < 0.01; ***, p < 0.001; by post-hoc t-test with Bonferroni correction for multiple comparisons. Phagocytosis data plotted as mean ± SEM