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. 2022 May 7;15:53. doi: 10.1186/s13045-022-01270-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characteristics of plasma tRF profiles in patients with NSCLC. A Scatter plot analysis revealed the differentially expressed tRFs between pre- and post-operation plasma samples. The red and blue points denote the significantly up- or down-regulated tRFs between two groups. B Length-wise distribution of tRFs between pre- and post-operation plasma samples in patients with NSCLC, showing the reads of most length-based tRF types in plasma were significantly decreased after removal of tumors. C, D Stacked plot for the number of tRFs and tiRNAs derived from the same tRNA: The X- and Y-axis represented the tRNAs and the number of different kinds of tRFs and tiRNAs derived from the same tRNA, respectively. E, F The pie charts indicated that tRF-5 is the major type of plasma tRFs in patients with NSCLC. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001