TABLE 2.
Linkers and primers used in this study
| Name | Designed C. glabrata ERG11 region (orientation) | Sequence (5′-3′)a |
|---|---|---|
| ERG11AF | 5′-end flanking (sense) | TTTTTTCCGCGG ATTTATCGCTTCTTCTCTAATCCA |
| ERG11AR | 5′-end flanking (antisense) | TTTTTTTCTAGA TGAGTATACGGGTTCTTCG |
| P5ERG11 | N terminus (sense) | TTTTTTGAATTC AATAACATGTCCACTGAAAAC |
| P3ERG11 | N terminus (antisense) | TTTTTTGTCGAC GTGACCCTTTGGACCCAAG |
| ERG11CH5 | 5′-end flanking (sense) | CCAACTACAATCGAGTGAGC |
| ERG11CH3 | N terminus (antisense) | TTCCATTAGTCTGTGGTTTGG |
a
Italic letters indicate restriction enzyme sites.