TABLE 3.
Effect of DOX and human serum on sterol composition of controllable strains and ATCC 2001 cellsa
| Strain | Incubation time (h) | Medium supplementation
|
Amt of sterol (μg/g [dry weight] of cell)
|
|||||
|---|---|---|---|---|---|---|---|---|
| DOX (μg/ml) | Fluconazole (μg/ml) | Serum (%) | Cholesterol | Ergosterol | 4,14-Dimethyl-zymosterol | Lanosterol | ||
| 97ERG11 | 8 | 0 | 0 | 0 | 0 | 180.6 ± 26.2 | 58.7 ± 7.0 | 221.0 ± 19.7 |
| 8 | 10 | 0 | 0 | 0 | 26.1 ± 3.2 | 359.8 ± 33.2 | 1405.8 ± 103.2 | |
| 99ERG11 | 8 | 0 | 0 | 0 | 0 | 963.6 ± 65.5 | 42.3 ± 6.4 | 25.2 ± 6.8 |
| 8 | 10 | 0 | 0 | 0 | 108.5 ± 12.3 | 337.6 ± 7.2 | 1201.2 ± 17.9 | |
| 98ERG11 | 8 | 0 | 0 | 0 | 0 | 277.5 ± 25.2 | 12.1 ± 2.9 | 12.1 ± 3.0 |
| 8 | 10 | 0 | 0 | 0 | 33.2 ± 2.3 | 376.7 ± 43.0 | 1380.1 ± 100.3 | |
| 8 | 0 | 0 | 5 | 1101.9 ± 118.9 | 671.4 ± 63.9 | 30.5 ± 2.4 | 41.8 ± 14.4 | |
| 8 | 10 | 0 | 5 | 1200.9 ± 172.4 | 45.5 ± 1.3 | 305.2 ± 38.4 | 1185.3 ± 159.7 | |
| ATCC 2001 | 8 | 0 | 0 | 0 | 0 | 301.4 ± 27.9 | 12.0 ± 0.6 | 5.9 ± 0.8 |
| 8 | 0 | 30 | 0 | 0 | 18.3 ± 1.6 | 183.7 ± 16.1 | 630.8 ± 46.5 | |
| 8 | 0 | 0 | 5 | 808.1 ± 43.3 | 254.5 ± 16.1 | 7.9 ± 2.5 | 5.0 ± 2.0 | |
| 98ERG11 | 16 | 0 | 0 | 0 | 0 | 591.1 ± 19.5 | 20.3 ± 1.4 | 11.2 ± 2.1 |
| 16 | 10 | 0 | 0 | 0 | 28.1 ± 2.8 | 246.8 ± 9.5 | 1202.0 ± 97.9 | |
| 16 | 0 | 0 | 5 | 93.0 ± 1.5 | 562.3 ± 33.1 | 22.6 ± 4.9 | 32.8 ± 16.2 | |
| 16 | 10 | 0 | 5 | 388.7 ± 19.8 | 45.9 ± 9.8 | 187.1 ± 11.2 | 906.0 ± 56.2 | |
| ATCC 2001 | 16 | 0 | 0 | 0 | 0 | 265.8 ± 12.3 | 10.2 ± 1.0 | 9.4 ± 2.2 |
| 16 | 0 | 0 | 5 | 48.4 ± 2.4 | 245.0 ± 6.3 | 8.3 ± 1.5 | 6.6 ± 0.6 | |
a
Values are means ± standard deviations (three independent samples per group).