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. 2022 May 7;15:52. doi: 10.1186/s13045-022-01272-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Knockdown of LncRNA-PACERR hinders the M2 polarization and pro-tumour functions of THP-1-derived TAMs in vitro. A qPCR analysis of the relative expression of M2 markers (Arginase-1, CD163, TGFβ, CD206, IL-10 and IL-6) and M1 marker (CD80, IL-1β) in THP-1-derived TAMs after LncRNA-PACERR knockdown. THP-1 cells were treated with PMA and co-cultured with PANC-1 cells for two days. Data are shown as the results from three independent experiments. B Flow cytometric analysis of the expression of M2 markers (CD163 and CD206) in THP-1-derived TAMs after LncRNA-PACERR knockdown. THP-1 cells were treated with PMA and co-cultured with PANC-1 cells for two days. Data are shown as the results from two independent experiments. C–E Proliferation (C), migration (D) and invasion (E) capacity of PATU-8988 or PANC-1 cells co-cultured with THP-1-derived TAMs (shNC/ shPACERR). shNC means that cells were transfected in negative control plasmids. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001