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. 2022 May 7;15:52. doi: 10.1186/s13045-022-01272-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — LncRNA-PACERR functions as a ceRNA to sponge miR-671-3p in TAMs. A Fluorescence in situ hybridization (FISH) of LncRNA-PACERR (green) in THP-1-derived TAMs (co-cultured with PATU-8988 or PANC-1). DAPI staining (blue) shows the nuclei. Scar bar: 10 μm. B, C Grey value of LncRNA-PACERR and DAPI on the FISH from THP-1 derived TAMs. Green represents LncRNA-PACERR. Blue represents DAPI. D Expression levels of LncRNA-PACERR in the cytoplasm and nucleus in THP-1-derived TAMs. E Expression of two potential target miRNAs (miR-671-3p and miR-4448) in THP-1 derived TAMs after LncRNA-PACERR knocked down. F, G Expression of pri-miR-671-3p (F) and pre-miR-671-3p (G) in THP-1 derived TAMs transfected with empty control or pcDNA-LncRNA-PACERR. H Promoter luciferase activity of miR-671-3p in 293-T cells overexpressing LncRNA-PACERR. I RIP assay was performed using rabbit AGO2 and IgG antibodies in THP-1 derived TAMs. Relative expression levels of LncRNA-PACERR and miR-671-3p were determined by qRT-PCR. J, K Dual luciferase activity in 293-T cells co-transfected with LncRNA-PACERR wild-type or mutant sequence and miR-671-3p mimics. L Expression of miR-671-3p in 46 pairs of TAMs and M1-NTRMs from PDAC patients. M Correlation analysis between LncRNA-PACERR and miR-671-3p using expression data from 46 pairs of TAMs. N Kaplan–Meier survival curve presenting the overall survival of 110 PDAC patients, grouped according to the extent of miR-671-3p⁺ TAMs infiltration