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. 2022 May 7;15:52. doi: 10.1186/s13045-022-01272-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — MiR-671-3p directly binds to the 3′UTR of KLF12 and regulates the KLF12/AKT/c-myc axis. A Predicted target genes of miR-671-3p using Starbase, miRWalk, miRbase, RNA22 and the two overlapping target genes (NMT-1 and KLF12). B Expression of KLF12 in 46 pairs of TAMs and M1-NTRMs from PDAC patients. C Correlation analysis between miR-671-3p and KLF12 using data from 46 pairs of PDAC patients. D mRNA expression of KLF12 in THP-1 derived TAMs (co-cultured with PATU-8988 or PANC-1 cells) transfected with empty control or miR-671-3p mimics or inhibitor. E, F Dual luciferase activity in HEK-293 T cells co-transfected with the KLF12 wild-type or mutant sequence (E) and miR-671-3p mimics. G Correlation analysis between LncRNA-PACERR and KLF12 in TAMs from 46 PDAC patients. H mRNA expression of KLF12 in THP-1 derived TAMs co-transfected with the LncRNA-PACERR knocked down vector and miR-671-3p inhibitor. I Protein levels of KLF12 in THP-1 derived TAMs (co-cultured with PATU-8988 or PANC-1 cells). J Dual luciferase activity in HEK-293 T cells co-transfected with LncRNA-PACERR overexpression vector and miR-671-3p mimics. K, L Potein expression of PI3K/KLF12/AKT/c-myc axis in THP-1 derived TAMs (co-cultured with PANC-1 (K) or PATU-8988 cells (L)). M LncRNA-PACERR expression was evaluated by qRT-PCR in THP-1 derived TAMs with indicated treatment. The data are presented as the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001