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. 2022 May 7;15:52. doi: 10.1186/s13045-022-01272-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — KLF12 binds directly to LncRNA-PACERR and recruits EP300 to the promoter region of LncRNA-PACERR in a LncRNA-PACERR-dependent manner. A Association of KLF12 with the promoter region of LncRNA-PACERR in THP-1-derived TAMs (Crtl/KLF12 OE / shKLF12) analysed by ChIP-qPCR. B ChIP assays showed endogenous KLF12 binding to the LncRNA-PACERR gene promoter. C, D HEK293T cells were co-transfected with LncRNA-PACERR promoter–luciferase truncations and KLF12 plasmids, and the luciferase activity was determined using a dual luciferase reporter assay after 48 h. E Dual luciferase assay of HEK293T cells co-transfected with firefly luciferase constructs containing the wild-type or mutant KLF12 potential binding sites of LncRNA-PACERR promoter and KLF12 plasmids were performed. F Association of H3K27ac with the promoter region of LncRNA-PACERR in THP-1-derived TAMs (Crtl/ KLF12 OE/ shKLF12) analysed by ChIP-qPCR. G Results of coimmunoprecipitation (Co-IP) in THP-1-derived TAMs. Normal rabbit IgG was used as a negative control. H WB validation of KLF12 proteins pulled down with biotin-labelled LncRNA-PACERR is shown. I RNA immunoprecipitation (RIP) was performed using a KLF12-specific antibody. Eluted KLF12-binding RNAs were reverse transcribed, and qPCR was performed with primers specific for LncRNA-PACERR. Normal rabbit IgG (IgG) was used as a negative control. Data are shown as the results from three independent experiments. J Association of EP300 with the promoter region of LncRNA-PACERR analysed by ChIP-qPCR in THP-1-derived TAMs (shNC/ shLncRNA-PACERR + KLF12 OE/shKLF12 + LncRNA-PACERR OE/ shKLF12 + shLncRNA-PACERR)