Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 7;15:52. doi: 10.1186/s13045-022-01272-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 7 — LncRNA-PACERR directly binds with IGF2BP2 in TAMs. A Visualization of protein bands by biotin-labelled LncRNA-PACERR RNA probes incubated with total protein extracts from THP-1 derived TAMs. B Immunoblotting to determine the specific association of IGF2BP2 with biotinylated LncRNA-PACERR. C qRT-PCR analysis of LncRNA-PACERR enriched by IGF2BP2 in THP-1 derived TAMs (top). Immunoblot of IGF2BP2 is shown (bottom). IP, immunoprecipitation. D Images showing the colocalization of LncRNA-PACERR and IGF2BP2 in the IGF2BP2 KD and LncRNA-PACERR KD THP-1 derived TAMs. Scale bars: 20 μm. E Secondary structure of LncRNA-PACERR analysed by RNAfold web server and deletion mapping of biotinylated LncRNA-PACERR motifs, as indicated. The red boxes represent the remaining fragments of LncRNA-PACERR, with the corresponding number label in the corner. F Immunoblot showing the association of IGF2BP2 with biotinylated LncRNA-PACERR RNA strands and the above-mentioned biotinylated LncRNA-PACERR motifs. G Schematic structures showing six domains in IGF2BP2. H RIP analysis for LncRNA-PACERR enrichment in HEK293T cells transfected with the FLAG-tagged full-length or truncated IGF2BP2 constructs (n = 3). aa: amino acid. I qRT-PCR analysis of IGF2BP2 mRNA levels (top) and immunoblot of IGF2BP2 (bottom) in the LncRNA-PACERR KD cells (n = 3). J qRT-PCR analysis of LncRNA-PACERR levels (top) and immunoblot of IGF2BP2 (bottom) in the IGF2BP2 KD cells (n = 3). The results are presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, ns not significant