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. 2022 May 7;22:124. doi: 10.1186/s12866-022-02524-y

Table 10.

Temperature and time of different stages of PCR

Final Extention Extention Anneling Denaturation Primery Cycle number Gene
denaturation
10Min/72 50 s / 72 50 s / 53 1Min/94 5Min/94 30 qac-E
10Min/72 50 s / 72 45 s / 51 1Min/94 5Min/94 30 qacE-Δ1
10Min/72 50 s / 72 50 s / 53 1Min/94 5Min/94 30 SUG-E1
10Min/72 50 s / 72 50 s / 57 1Min/94 5Min/94 30 ZntB