Fig. 2.

Detection of CRISPR/Cas9-mediated nucleotide variations in founder animals. A Schematic diagram of the MSTN gene structure and targeting loci of chosen sgRNAs. sgRNAs targeting sites are highlighted in yellow; protospacer adjacent motif (PAM) sequences are highlighted in red. B Representative images of a 30-day-old MSTN gene-edited (Mut) and wild-type (WT) lambs. C Genotypes of target sites in eight founder animals as determined by targeted deep sequencing. Mutations are highlighted in blue and (−) indicates deletions