Figure 3.

Number and morphology of photoreceptors in control and sodium hyaluronate-treated eyes. (A–D) Cross-sections of the central retina from control (A, C) and HYA-treated (B, D) eyes immunostained for cone arrestin (A, B, cone photoreceptors, green) or cone arrestin and Bassoon (C, D, cone photoreceptors, green; synaptic ribbon, red). The nuclear marker TO-PRO 3 iodide (blue) was used to visualize all cell nuclei. Insets show a magnification of cone pedicles and synaptic ribbons (C, D). Arrowheads point to synaptic ribbons inside cone pedicles. (E-G) Quantification of photoreceptor rows in ONL (E), cones per millimeter of retinal section (F) and INL thickness (G) in both control and HYA-treated eyes. ANOVA, Bonferroni's test, *P < 0.05, n = 7 in each group. ONL, outer nuclear layer; ON, optic nerve. Scale bar: 20 µm (A–D), 5 µm (C, D insets).