KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| mouse anti-Prospero | DSHB | MR1A, RRID: AB_528440 |
| mouse anti-Delta | DSHB | C594.9B, RRID: AB_528194 |
| chicken anti-GFP | Thermo Fisher | A10262, RRID: AB_2534023 |
| rabbit anti-Relish | RayBiotech | ABIN1111036, RRID: AB_11219024 |
| rabbit anti-Sox21a | Benoit Biteau | Meng and Biteau, 2015 |
| and rabbit anti-PH3 | EMD Millipore | 06-570, RRID: AB_310177 |
| donkey anti-mouse-555 | Thermo Fisher | A31570, RRID: AB_2536180 |
| donkey ant-rabbit-555 | Thermo Fisher | A31572, RRID: AB_162543 |
| donkey anti-mouse-647 | Thermo Fisher | A31571, RRID: AB_162542 |
| donkey anti-rabbit-647 | Thermo Fisher | A31573, RRID: AB_2536183 |
| goat anti-chicken-488 | Thermo Fisher | A11039, RRID: AB_142924 |
| Bacterial and virus strains | ||
| Erwinia carotovora ssp. carotovora 15 | This strain is available upon request | N/A |
| Pseudomonas entomophila | This strain is available upon request | N/A |
| Vibrio cholerae | This strain is available upon request | N/A |
| Acetobacter tropicalis | This strain is available upon request | N/A |
| Lactobacillus plantarum | This strain is available upon request | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| DAPI | Sigma-Aldrich | D9564 |
| Alexa Fluor 555 Phalloidin | Thermo Fisher | R415 |
| Citifluor AF1 | EMS | 17970-100 |
| Elastase | Sigma-Aldrich | E0258 |
| DSS | EMD Millipore | 80502-348 |
| Bleomycin | Enzo Life Sciences | 89156-580 |
| 20% paraformaldehyde | EMS | 15713 |
| Grape agar powder | Flystuff | 47-102 |
| Trizol | Life Technologies | 15596018 |
| Critical commercial assays | ||
| RNeasy Mini kit | Qiagen | 74106 |
| Quantseq 3′ mRNA-Seq | Lexogen | 015.24 |
| qScript cDNA Synthesis Kit | Quanta | 95150-01K |
| PerfeCTa SYBR Green FastMix | Quanta | 95047-100 |
| DNeasy Blood and Tissue kit | Qiagen | 69506 |
| Deposited data | ||
| DamID | this paper | SRA: PRJNA736303 |
| RNAseq | this paper | SRA: PRJNA757772 SRA: PRJNA779708 |
| Experimental models: Organisms/strains | ||
| Hikone-A-W | Bloomington Drosophila Stock Center | #4 |
| Oregon-R-P2 | Bloomington Drosophila Stock Center | #2376 |
| DGRP-391 | Bloomington Drosophila Stock Center | #25191 |
| DGRP-859 | Bloomington Drosophila Stock Center | #25210 |
| RelE20 | Hedengren et al., 1999 | N/A |
| ImdR156 | Hedengren et al., 1999 | N/A |
| PGRP-LE112/LCE12 | Takehana et al., 2004 | N/A |
| PGRP-LB KO | Paredes et al., 2011 | N/A |
| tub-Gal80TS; Da-Gal4 | Bloomington Drosophila Stock Center | #86326 |
| Esg-Gal4/CyOlacZ; UAS-GFP, tub-Gal80TS | Jiang et al., 2009 | N/A |
| Su(H)GBE-Gal4; UAS-GFP, tub-Gal80TS | Jiang et al., 2009 | N/A |
| UAS-GFP, tub-Gal80TS; prosv1-Gal4 / TM6B | Zeng et al., 2010 | N/A |
| UAS-Imd | Georgel et al., 2001 | N/A |
| UAS-Rel | Georgel et al., 2001 | N/A |
| UAS-RasV12 | Bloomington Drosophila Stock Center | #4847 |
| UAS-Dome-DN | Harrison et al., 1995 | N/A |
| UAS-HopTuml | Harrison et al., 1995 | N/A |
| UAS-nls-GFP | Bloomington Drosophila Stock Center | #4775 |
| TI{2A-GAL4}AstC[2A-GAL4] | Bloomington Drosophila Stock Center | #84595 |
| TI{2A-GAL4}Tk[2A-GAL4] | Bloomington Drosophila Stock Center | #84693 |
| UAS-Rel-IR | Bloomington Drosophila Stock Center | #33661, #28943 |
| UAS-PGRP-LE-IR | Bloomington Drosophila Stock Center | #60038 |
| UAS-PGRP-LC-IR | Bloomington Drosophila Stock Center | #33383 |
| UAS-Notch-IR | Bloomington Drosophila Stock Center | #33611 |
| UAS-EGFR-IR | Bloomington Drosophila Stock Center | #25781 |
| Esg-Gal4,Ubi-p63e-loxP-stop-loxp-GFP | Zeng and Hou, 2015 | N/A |
| Su(H)GBE-Gal4,Ubi-p63e-loxP-stop-loxP-GFP | Zeng and Hou, 2015 | N/A |
| UAS-RedStinger, UAS-FLP, Ubi-p63(FRT.STOP)Stinger | Bloomington Drosophila Stock Center | #28280 |
| yw; FRT40A UAS CD8 RFT, UAS CD2-Mir / CyO; tubGal4, tubgal80ts /TM6C | Yu et al., 2009 | N/A |
| hsflp; FRT40A UAS CD2 RFT, UAS GFP-Mir/CyO | Yu et al., 2009 | N/A |
| Oligonucleotides | ||
| Fw primer (Dam-Rel cloning): GCGGCCGCGGATGAACATGAATCAGTACTACG | this paper | N/A |
| Rv primer (Dam-Rel cloning): CGGCCGCAGTCAAGTTGGGTTAACCAGTAG | this paper | N/A |
| Dpt Fw: GCTGCGCAATCGCTTCTACT | (Troha et al., 2019) | N/A |
| Dpt Rv: TGGTGGAGTGGGCTTCATG | (Troha et al., 2019) | N/A |
| Rpl32 Fw: GACGCTTCAAGGGACAGTATCTG | (Troha et al., 2019) | N/A |
| Rpl32 Rv: AAACGCGGTTCTGCATGAG | (Troha et al., 2019) | N/A |
| Software and algorithms | ||
| R Studio | RStudio Team (2020) | N/A |
| Adobe Photoshop (23.1.1) | Adobe | N/A |
| Integrative Genomics Viewer (V2.5.3) | N/A | |
| ImageJ | NIH | N/A |