| Excitation wavelength maximum/λex (nm) |
200–700 |
319 |
| Emission wavelength maximum/λem (nm) |
200–700 |
373 |
| Solvent/amount of absolute ethanol/mL |
0–6 |
1–5 (preferably 2) |
| Acidity/M H2SO4
|
0.001–0.075 |
0.0125–0.05 (preferably 0.025) |
| pH |
3.69–0.69 |
3.0–1.0 (preferably 1.3) |
| Time/h |
0–72 |
1 min–24 h (preferably 5 min) |
| Temperature/°C |
10–70 |
15–40 (preferably 25 ± 5) |
| Reagent (fold molar excess, M : R) |
1 : 1–1 : 500 |
1 : 70–1 : 300 (preferably 1 : 100) |
| Linear range/μg L−1
|
0.001–1000 |
0.01–800 |
| Limit of quantification/ng L−1
|
1–100 |
10 |
| Detection limit/ng L−1
|
0.01–10 |
1 |
| Reproducibility (% RSD) |
0–10 |
0–2% |
| Regression co-efficient (R2) |
0.9992–0.9999 |
0.9998 |
