Fig. 6. Effect of the molar ratio of AMA to EDTA on NDM-1 inhibition. The molar ratio of AMA to EDTA (A), inhibition (%) = enzyme activity of NDM-1 (in the absence of inhibitor) – the enzyme activity (after 30 min with inhibitor addition), inhibitor final concentration was 10 μM. NDM-1 activity curve with time along with the time in the absence or in the presence of different concentrations of each inhibitors (B). The IC50 of different inhibitors (C). Imipenem hydrolysis inhibition in E. coli cells producing NDM-1 by AMA : EDTA = 1 : 2 (D), (
) control (no inhibitor), (
) adding inhibitor (AMA : EDTA = 1 : 2).
