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| Run | Factors | A initial cell density | B induction period (h) | C IPTG (mM) | NDM-1 activity (U ml−1) | ||
|---|---|---|---|---|---|---|---|
| A | B | C | |||||
| 1 | 1 | 1 | 1 | 0.5 | 15 | 0.55 | 18 |
| 2 | 1 | 2 | 2 | 0.5 | 20 | 0.65 | 67 |
| 3 | 1 | 3 | 3 | 0.5 | 25 | 0.75 | 28 |
| 4 | 2 | 1 | 2 | 0.6 | 15 | 0.65 | 43 |
| 5 | 2 | 2 | 3 | 0.6 | 20 | 0.75 | 52 |
| 6 | 2 | 3 | 1 | 0.6 | 25 | 0.55 | 60 |
| 7 | 3 | 1 | 3 | 0.7 | 15 | 0.75 | 48 |
| 8 | 3 | 2 | 1 | 0.7 | 20 | 0.55 | 23 |
| 9 | 3 | 3 | 2 | 0.7 | 25 | 0.65 | 34 |
| k1 | 37.67 | 36.33 | 33.67 | ||||
| k2 | 51.67 | 47.33 | 48.00 | ||||
| k3 | 35.00 | 40.67 | 42.67 | ||||
| Delt | 16.67 | 11.00 | 14.33 | ||||
| Ran | 1 | 3 | 2 | ||||
